BD LSRII (L01/L02) 1.0 Purpose The purpose of this procedure is to provide a comprehensive and consistent approach to using the BD™ High Throughput Sampler (HTS; Plate Loader) on the DHVI Flow Facility L01 and L02 bench-top analyzers. 2.0 Scope and Application This SOP applies specifically to the BD LSRII (L01/L02) instruments in the

14 Jul 2020 Fortessa, Verse, Aria from BD Biosciences), prospectively on blood freshly collected “Manual” compensation of the flow cytometry data files.

The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa™ cell analyzer offers the ultimate choice for flow cytometry. Designed for customization, it has the ability to meet the needs of your multicolor flow cytometry assays.

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Simpler Gating Features † Proportional resizing of polygon and rectangular gates. See Editing Gates on page 48 in this guide. † Drag and drop gates within a plot or from one plot to another within a worksheet. See Dragging and Dropping Gates into Plots on page 232 in the BD FACSDiva Software Reference About This Manual This manual describes how to use BD FACSDiva™ software.

Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential.

• Select a well or group of wells, and then click the Add Setup Controls button . • Select a well or group of wells, and then click the Add Specimen Wells button . • The loader (HTS) settings can be modified for the selected well or plate. The LSR Fortessa uses BD FacsFlow as the sheath fluid.

Fortessa Door Handles are covered by a 10 Year Guarantee on mechanical parts. Our levers have been tested to pass DIN EN 1906 European Quality Standards, and have been tested for frequency of use. Essentially each door handle is guaranteed for 100,000 revolutions.

The BD LSRFortessa 1 cell analyzer is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has three spatially separated lasers - 405 nm, 488 nm and 640 nm. BD Fortessa The BD Fortessa cell analyzer provides the flexibility to support measurement of multicolor flow cytometry assays of up to 16 parameters at a time. The FCF is equipped with one BD LSRFortessa™ (Diagnostic Purpose) and a BD LSRFortessa™ X-20 (Research Purpose). 2019-09-13 · Utah Flow Cytometry Core Facility demonstrates how to switch probes on the BD FORTESSA analyzer. Fortessa Door Handles are covered by a 10 Year Guarantee on mechanical parts. Our levers have been tested to pass DIN EN 1906 European Quality Standards, and have been tested for frequency of use.
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Additionally, the challenges of successful multi-parameter analysis in terms of antibody-fluorochrome selection, instrument setup BD FACSFlow™’Supply’System’Fluidics’Cart computer, wait 1 minute, then start up the LSR Fortessa and try to connect again in FACSDiva.

Fortessa X-20 Cell Analyzer. Manufacturer: BD Bioscience Location: PSC B6 Supporting Documents: Instrument Manual. University of Southern California 1985 Zonal Avenue PSC B5, B6, 210BB, 402B-G, 510, 603 and 612 (Please see individual instruments for their location.) Los Angeles, CA 90089-9121 .
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BD LSR Fortessa Standard Operation Protocol --- Turn on and turn ong>BD ong> ong>LSR ong> ong>Fortessa ong> ong>Standard ong> ong>Operation ong> ong>Protocol ong>--- ong>Turn BD LSR II Us

BD HTS Instruction manual. Scheduling. Please contact us to schedule time on the instrument, set up a training appointment or to discuss a future project: flowcyt@illinois.edu, (217) 244-0559. The LSR Fortessa uses BD FacsFlow as the sheath fluid. 20 L boxes of which are available in lab 1.47a (CIMR) and on the bench behind the machine (e6) Please contact Flow Core Unit staff if none are present The sheath fluid sits on the left of the FACS Flow Supply cart underneath the bench.